Efficient methods and apparatus for high-throughput processing of gene sequence data
US7110885B2 · kind B2 · utility
Assignee
Inventor
Key dates
| Filing date | Sep 26, 2001 |
| Grant date | Sep 19, 2006 |
| Priority date | — |
| Expiry date | Jan 14, 2023 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG16B30/00
- WIPO fieldComputer technology
- WIPO sectorElectrical engineering
Abstract
One disclosed method of processing gene sequence data includes the steps of reading gene sequence data corresponding to a gene sequence and coding sequence data corresponding to a plurality of coding sequences within the gene sequence; identifying and storing, by following a set of primer selection rules, primer pair data within the gene sequence data for one of the coding sequences; repeating the acts of identifying and storing such that primer pair data are obtained for each sequence of the plurality of coding sequences; and simultaneously amplifying the plurality of coding sequences in gene sequences from three or more of individuals using the identified pairs of primer sequences. The set of primer selection rules include a rule specifying that all of the primer pair data for the plurality of coding sequences be obtained for a predetermined annealing temperature, which allows for the subsequent simultaneous amplification of sequences from hundreds of individuals in a single amplification run.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.