Process for high throughput DNA methylation analysis

US7112404B2 · kind B2 · utility

Assignee

• EPIGENOMICS AG · DE

Inventors

• Peter W. Laird · South Pasadena, US
• Cindy Eads · South Pasadena, US
• Kathleen Danenberg · Altadena, US

Key dates

• Filing date: Dec 10, 2001
• Grant date: Sep 26, 2006
• Expiry date: Dec 10, 2021

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/6858
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

There is disclosed an improved high-throughput and quantitative process for determining methylation patterns in genomic DNA samples based on amplifying modified nucleic acid, and detecting methylated nucleic acid based on amplification-dependent displacement of specifically annealed hybridization probes. Specifically, the inventive process provides for treating genomic DNA samples with sodium bisulfite to create methylation-dependent sequence differences, followed by detection with fluorescence-based quantitative PCR techniques. The process is particularly well suited for the rapid analysis of a large number of nucleic acid samples, such as those from collections of tumor tissues.