Nucleic acid amplification using nicking agents
US7112423B2 · kind B2 · utility
100Cited by
47References
16Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Jul 15, 2002 |
| Grant date | Sep 26, 2006 |
| Priority date | — |
| Expiry date | Jan 7, 2023 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC40B40/06
- WIPO fieldOrganic fine chemistry
- WIPO sectorChemistry
Abstract
The present invention provides methods and kits for amplifying target nucleic acids (including whole genomes) using nicking agents. In certain aspects, the amplification does not require the use of any external oligonucleotide primers that are capable of annealing to a portion of the target nucleic acid. This invention is useful in many areas such as genetic disease diagnoses, forensic analyses and palcoarcheological studies.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.