Cell-based assays for G-protein-coupled receptor-mediated activities
US7115377B2 · kind B2 · utility
14Cited by
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55Claims
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Key dates
| Filing date | Mar 4, 2002 |
| Grant date | Oct 3, 2006 |
| Priority date | — |
| Expiry date | Jul 14, 2022 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2500/10
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Disclosed are compositions and methods for their use, such as in identifying G-protein-coupled receptors, ligands and compounds that modulate the activities of G-protein-coupled receptors. The compositions and methods employ cyclic nucleotide-gated channels and fluorescence dyes in detecting changes of intracellular cAMP levels in response to the stimulation of G-protein-coupled receptors. Activation of the G-protein-coupled receptors can be detected in a variety of assays, including cell-based imaging assays with fluorescence microscopes and high throughput assays with multi-well plates and fluorescence plate readers.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.