Recombinant type II restriction endonucleases, MmeI and related endonucleases and methods for producing the same
US7115407B2 · kind B2 · utility
3Cited by
2References
6Claims
0Family size
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Key dates
| Filing date | Jul 10, 2003 |
| Grant date | Oct 3, 2006 |
| Priority date | — |
| Expiry date | May 30, 2024 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/52
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
In accordance with the present invention, there is provided a DNA (deoxyribonucleic acid) fragment which encodes the MmeI type II restriction endonuclease enzyme. This one polypeptide possesses two related enzymatic functions; namely an endonuclease activity which recognizes the DNA sequence 5′-TCC(Pu)AC-3′ and cleaves as indicated by the arrows:and a second enzymatic activity that recognizes the same DNA sequence, 5′-TCC(Pu)AC-3′, but modifies this sequence by the addition of a methyl group to prevent cleavage by the MmeI endonuclease activity.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.