Near-field intra-cellular apertureless microscope

US7116475B2 · kind B2 · utility

Assignee

• Nanopoint, Inc.

Inventors

• Daniel G. O'Connell · Kihei, US
• Caitlin E. O'Connell-Rodwall · Menlo Park, US

Key dates

• Filing date: Jun 18, 2003
• Grant date: Oct 3, 2006
• Expiry date: Aug 28, 2023

Classification

• Technology area (CPC G): Physics
• CPC primary: G01Q60/20
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Sub-wavelength size fluorescent particles attach to specific gene sites or a magnetic bead that is maneuvered around a cell volume to produce evanescent fields when illuminated in the far-field from light outside the cell volume. Light scattering from the sub-wavelength particles produces near-field interactions with surrounding molecules. The sub-wavelength scattering particles may be metallic spheres. Using particles within the cell removes large far-field scattered light from the mechanical structure of a supporting probe. Near-field light is modulated with an oscillating magnetic field, and micro-positioning is accomplished by a computer controlled DC magnetic field to scan the particle around within the cell. The Near-Field Intra-Cellular Apertureless Microscope (NICAM) technique enables non-destructive sub-wavelength resolution imaging without inserting a near-field (illumination or collection mode) probe into a cell.