Method for detecting cytosine methylations
US7118868B2 · kind B2 · utility
Assignee
Inventor
Key dates
| Filing date | Jun 19, 2001 |
| Grant date | Oct 10, 2006 |
| Priority date | — |
| Expiry date | Jul 22, 2022 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q2533/101
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method is described for the detection of 5-methylcytosine in genomic DNA samples. First, a genomic DNA from a DNA sample is chemically converted with a reagent, whereby 5-methylcytosine and cytosine react differently. Then the pretreated DNA is amplified with the use of a polymerase with primers of different sequence. In the next step, the amplified genomic DNA is hybridized to an oligonucleotide array and PCR products are obtained, which must be provided with a label. Alternatively, the PCR products can be extended in a primer extension reaction, wherein the extension products are also provided with a label.In the last step, the extended oligonucleotides are investigated for the presence of the label.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.