Recombinant bovine pancreatic desoxyribonuclease I with high specific activity

US7118901B2 · kind B2 · utility

Assignee

• Roche Diagnostics Operations, Inc. · US

Inventors

• Bernhard Suppmann · Weilheim, DE
• Johann-Peter Thalhofer · Weilheim, DE
• Stephanie Ronning · Penzberg, DE
• Helmut Schoen · Penzberg, DE
• Rainer Muller · Penzberg, DE

Key dates

• Filing date: Dec 16, 2003
• Grant date: Oct 10, 2006
• Expiry date: Aug 11, 2024

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N9/22
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A method is provided for the production of a bovine pancreatic protein with a specific desoxyribonuclease activity of at least 6,000 units per mg of protein. Methylotrophic yeast is used as a heterologous host organism. The bovine pancreatic protein is secreted into the growth medium from which it is purified.