Patent · US Expired

Composition and method for hot start nucleic acid amplification

US7122355B2 · kind B2 · utility

9Cited by

20References

8Claims

0Family size

Assignee

Roche Diagnostics Operations, Inc. · US

Inventors

Waltraud Ankenbauer · Penzberg, DE
Dieter Heindl · Munich, DE
Frank Laue · Kempten (Allgäu), DE
Andreas R. Huber · Aarau, CH

Key dates

Filing date	Jul 11, 2002
Grant date	Oct 17, 2006
Priority date	—
Expiry date	Jun 21, 2023

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12Q1/6853
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

The present invention is directed to a new composition for performing a nucleic acid amplification reaction comprising (i) a thermostable DNA-Polymerase, (ii) a thermostable 3′-5′ Exonuclease, and (iii) at least one primer for nucleic acid amplification with a modified 3′ terminal residue which is not elongated by said thermostable DNA-Polymerase as well as methods for performing a PCR reaction using this composition. Furthermore, the method is directed to kits comprising such a composition.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.