Additives for reversed-phase HPLC mobile phases
US7125492B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Jul 17, 2003 |
| Grant date | Oct 24, 2006 |
| Priority date | — |
| Expiry date | Jul 17, 2023 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2030/342
- WIPO fieldChemical engineering
- WIPO sectorChemistry
Abstract
The present invention provides silica-based reversed-phase HPLC methods that lead to higher retention of the analytes in the column and longer column lifetimes than usually observed under medium to high pH aqueous mobile phase conditions. The inventive methods comprise eluting the HPLC column using an aqueous mobile phase comprising at least one fluorinated additive. Preferred additives are polyfluorinated alcohols such as 2,2,2-trifluoroethanol and 1,1,1,3,3,3-hexafluoroisopropanol. The methods of the present invention may be used for analyzing, separating, purifying, and/or isolating small organic molecules, natural products, as well as biomolecules such as polypeptides, oligonucleotides and polynucleotides (e.g., DNA fragments).
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.