Solid-phase immobilization of proteins and peptides
US7125669B2 · kind B2 · utility
Assignee
Inventor
Key dates
| Filing date | Nov 21, 2002 |
| Grant date | Oct 24, 2006 |
| Priority date | — |
| Expiry date | Apr 1, 2024 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K17/06
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Disclosed herein are methods for immobilizing a peptide or protein on a solid support. The method generally includes the following steps: (a) providing one or more templates attached to a solid support, wherein the one or more templates include (i) an RNA encoding a peptide and (ii) a peptide acceptor-linker linked to the RNA; and (b) subjecting the one or more templates to conditions that support translation and attachment of said peptide to said peptide acceptor, thereby synthesizing the one or more peptides on the solid support. Also disclosed herein are solid supports having at least one RNA-protein fusion component immobilized thereon, methods for generating protein arrays, and methods for screening molecules using these arrays.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.