Patent · US Expired

Methods to screen peptide libraries using minicell display

US7125679B2 · kind B2 · utility

35Cited by
11References
20Claims
0Family size

Assignee

Inventor

Key dates

Filing dateMar 6, 2002
Grant dateOct 24, 2006
Priority date—
Expiry dateJul 12, 2023

Classification

  • Technology area (CPC C)Chemistry; Metallurgy
  • CPC primaryC07K2319/02
  • WIPO fieldOrganic fine chemistry
  • WIPO sectorChemistry

Abstract

A minicell display method has been developed which has significant advantages for screening peptide libraries for candidates that can bind and effectively modulate a particular biological process. The method, based on the small, anucleate minicell, has increased versatility in generating unique sequences to screen as well as increasing the size of the peptides to be screened. In vivo mutagenesis, at the level of protein synthesis, as well as DNA replication, increases diversification of the library to be screened and therefore substantially increases the number of potential peptides that can modulate a particular biological response or mechanism.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.