Reporter molecules and methods for assaying the lipidations status of a cell
US7129036B1 · kind B1 · utility
Assignee
Inventors
Key dates
| Filing date | May 26, 2000 |
| Grant date | Oct 31, 2006 |
| Priority date | — |
| Expiry date | May 26, 2020 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2333/82
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The invention relates to reporter molecules comprising at least one detection domain capable of emitting a signal and at least one membrane anchoring domain, which promotes the association of the reporter molecule with a membrane. The degree and rate of membrane association of the reporter molecule is approximated by the signal emitted by the detection domain. This signal provides a marker for the membrane association status of the membrane anchoring portion, or of the reporter molecule as a whole. Treatments or agents may be tested for their ability to alter membrane association or localization by observing or measuring the signal emitted by the detection domain. Thus, the invention provides means and methods for determining the capability of an agent to interfere at least in part with the distribution of a reporter molecule in a cell. These methods are useful for drug discovery; the phenotypic evaluation of cells, preferably patient cells; and for tailoring a therapeutic program for treating a patient.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.