Oligonucleotide-facilitated coalescence

US7129087B2 · kind B2 · utility

Assignee

• The Public Health Research Institute Of the City of New York, Inc. · US

Inventors

• Fred R. Kramer · Yonkers, US
• Osama A. Alsmadi · Guilford, US
• Sanjay Tyagi · New York, US

Key dates

• Filing date: Oct 11, 2001
• Grant date: Oct 31, 2006
• Expiry date: Jul 2, 2022

Classification

• Technology area (CPC A): Human Necessities
• CPC primary: A61P43/00
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

Coalescence of cells or other membrane-bound entities is facilitated by anchoring an outwardly projecting first oligonucleotide in one member and an outwardly projecting second oligonucleotide, complementary to the first, in a second member and incubating under hybridizing conditions. Liposomes may be coalesced with cells to deliver hydrophilic agents thereto, such as DNA probes or drugs. Kits containing complementary oligonucleotides containing hydrophobic anchoring moieties may be used.