Forms of soluble pyrroloquinoline quinone-dependent glucose dehydrogenase

US7132270B2 · kind B2 · utility

Assignee

• Roche Diagnostics GMBH · DE

Inventors

• Peter Kratzsch · Penzberg, DE
• Rainer Schmuck · Benediktbeuern, DE
• Daniela Beck · Penzberg, DE
• Zhixin Shao
• Detlef Thym · Mannheim, DE
• Wolfgang-Reinhold Knappe · Ludwigshafen, DE

Key dates

• Filing date: Dec 13, 2002
• Grant date: Nov 7, 2006
• Expiry date: Dec 20, 2022

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N9/0006
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present invention relates to improved variants of soluble pyrroloquinoline quinone (PQQ)-dependent glucose dehydrogenases (s-GDH), to genes encoding mutated s-GDH, to mutant proteins of s-GDH with improved substrate specificity for glucose, and to different applications of these s-GDH variants, particularly for determining concentrations of sugar, especially of glucose in a sample.