Primer extension methods for production of high specific activity nucleic acid probes
US7135284B1 · kind B1 · utility
7Cited by
10References
23Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Feb 4, 2000 |
| Grant date | Nov 14, 2006 |
| Priority date | — |
| Expiry date | Feb 4, 2020 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6806
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention provides novel nucleic acid labeling techniques that generate nucleic acid probes with specific activities at least ten fold higher than the levels obtained using standard labeling methods. Specifically, the methods of the invention provides methods of producing nucleic acid probes that each comprises multiple labeled nucleotides. The methods can be used to generate RNA, DNA or hybrid probes. The invention also provides reaction mixtures and kits for the practice of the methods of the invention and compositions comprising the probes generated according to the methods of the invention.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.