Insect expression vectors
US7135337B2 · kind B2 · utility
Inventors
Key dates
| Filing date | Jun 29, 2001 |
| Grant date | Nov 14, 2006 |
| Priority date | — |
| Expiry date | Nov 16, 2021 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2840/20
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention provides insect shuttle vectors, and methods of using such vectors, for stably transforming disparate insect cell lines to express heterologous proteins. The invention provides a transformed insect cell selection system based on resistance to the bleomycin/phleomycin family of antibiotics, including the antibiotic Zeocin. Efficient promoters derived from baculovirus immediate early promoters are disclosed for use in directing expression of heterologous proteins, including selectable markers, in transformed insect cells of the invention. Transposon-based vectors are disclosed that provide inducible transposition to optimize heterologous protein expression and unobtrusive markers to facilitate selection of desired transformants.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.