Synthesis of polynucleotides using combined oxidation/deprotection chemistry

US7135565B2 · kind B2 · utility

Assignees

• AGILENT TECHNOLOGIES, INC. · US
• The Regents of the University of Colorado · US

Inventors

• Douglas J. Dellinger · Boulder, US
• Michael Perbost · Cupertino, US
• Marvin H. Caruthers · Boulder, US
• Jason Richard Betley · Stowmarket, GB

Key dates

• Filing date: Jul 27, 2001
• Grant date: Nov 14, 2006
• Expiry date: Sep 25, 2021

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y02P20/55
• WIPO field: Organic fine chemistry
• WIPO sector: Chemistry

Abstract

A method of synthesizing a polynucleotide which can, for example, be used during fabrication of an array. A second nucleoside is coupled to a first nucleoside through a phosphite linkage, with the second nucleoside having a hydroxyl protecting group that is a non-carbonate protecting group. The product of the foregoing step is exposed to a composition which both oxidizes the formed phosphite to a phosphate and deprotects the protected hydroxyl of the coupled nucleoside. The method has particular application to fabricating an addressable array of polynucleotides on a substrate which carries substrate bound moieties each with a hydroxyl group.