Methods for identifying or screening for agents that modulate 17β-HSD3
US7138246B2 · kind B2 · utility
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Key dates
| Filing date | Feb 28, 2005 |
| Grant date | Nov 21, 2006 |
| Priority date | — |
| Expiry date | Feb 28, 2025 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/743
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Methods for determining whether a test agent inhibits a 17β-HSD3, the methods comprising: obtaining a recombinant host cell that expresses the 17β-HSD3; obtaining a reaction mixture comprising the 17β-HSD3, androstenedione, and the test agent; measuring the amount of testosterone in the reaction mixture by a scintillation proximity assay (SPA) using SPA beads conjugated with a testosterone-specific antibody, wherein when the amount of testosterone is lower in the presence of a test agent than in the absence of the test agent, the test agent is identified as an inhibitor of the 17β-HSD3.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.