Masking of the background fluorescence and luminescence in the optical analysis of biomedical assays

US7138280B2 · kind B2 · utility

Assignee

• BAYER HEALTHCARE LLC · DE

Inventors

• Thomas Krahn · Hagen, DE
• Wolfgang Paffhausen · Leverkusen, DE
• Andreas Schade · Lengede, DE
• Martin Bechem · Wuppertal, DE
• Delf Schmidt · Wuppertal, DE

Key dates

• Filing date: Oct 3, 2002
• Grant date: Nov 21, 2006
• Expiry date: Oct 3, 2022

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10T436/13
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

In a process for the quantitative optical analysis of biological cells labelled with a fluorescent dye, the sensitivity of analytical detection can be considerably improved if a masking dye, which absorbs the excitation light for the fluorescent dye and/or its emission light is added to the solution surrounding the biological cells and/or if a separating layer permeable to the solution and absorbing and/or reflecting the excitation light or the emission light is applied to a layer of the biological cells at the bottom of a reaction vessel. This process can also be used for improving the sensitivity in the quantitative optical analysis of a luminescent biological cell layer. Analogously, these process principles can also be used in receptor studies for the masking of the interfering background radiation in the quantitative optical analysis of fluorescently or luminescently labelled reaction components.