Factor VIII:C nucleic acid molecules

US7138505B1 · kind B1 · utility

Assignee

• Novartis Vaccines and Diagnostics, Inc. · US

Inventors

• George Kuo · San Francisco, US
• Frank R. Masiarz · San Francisco, US
• Martha Truett · Oakland, US
• Pablo D. T. Valenzuela · San Francisco, US
• Mirella E. Rasmussen · Copenhagen, DK
• Daniel Caput · San Francisco, US
• Rae Lyn Burke · San Francisco, US
• Carol Pachl · Corona, US

Key dates

• Filing date: Aug 13, 1990
• Grant date: Nov 21, 2006
• Expiry date: Aug 13, 2010

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K14/755
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Methods and compositions are provided for recombinant DNA production of Factor VIIIC and truncated derivatives thereof. Based on amino acid sequences, probes are developed for isolating messenger RNA, cDNA and/or chromosomal DNA encoding for Factor VIIIC. The Factor VIIIC gene in its entirety, a fragment thereof, or a cDNA is then used for expression of Factor VIIIC in a host.The bacteriophage λFVIII23D containing the 14.43 kb EcoRI fragment was deposited at the A.T.C.C. on Jan. 4, 1984 and given Accession No. 40094. Also, the vector pSVF8-200 was deposited at the A.T.C.C. on Jul. 17, 1985 and given Accession No. 40190.