Transgenic animals
US7138562B2 · kind B2 · utility
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21Claims
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Assignee
Inventors
Key dates
| Filing date | Jul 18, 2001 |
| Grant date | Nov 21, 2006 |
| Priority date | — |
| Expiry date | Jul 18, 2021 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2740/13051
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention provides improved methods and compositions for the generation of transgenic non-human animals. The present invention permits the introduction of exogenous nucleic acid sequences into the genome of unfertilized eggs (e.g., pre-maturation oocytes and pre-fertilization oocytes) by microinjection of infectious retrovirus into the perivitelline space of the egg. The methods of the present invention provide an increased efficiency of production of transgenic animals with a reduced rate of generating animals which are mosaic for the presence of the transgene.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.