Methods and means for obtaining modified phenotypes
US7138565B2 · kind B2 · utility
33Cited by
5References
17Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | May 23, 2002 |
| Grant date | Nov 21, 2006 |
| Priority date | — |
| Expiry date | Jan 24, 2023 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Y302/01031
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Methods and means are provided for reducing the phenotypic expression of a nucleic acid of interest in eukaryotic cells, particularly in plant cells, by providing aberrant, preferably unpolyadenylated, target-specific RNA to the nucleus of the host cell. Preferably, the unpolyadenylated target-specific RNA is provided by transcription of a chimeric gene comprising a promoter, a DNA region encoding the target-specific RNA, a self-splicing ribozyme and a DNA region involved in 3′ end formation and polyadenylation.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.