Reagents and procedures for high-specificity labeling

US7141655B2 · kind B2 · utility

Assignee

• Rutgers, the State University of New Jersey · US

Inventors

• Richard H. Ebright · New Brunswick, US
• Yon W. Ebright · New Brunswick, US

Key dates

• Filing date: Jun 13, 2003
• Grant date: Nov 28, 2006
• Expiry date: Oct 28, 2024

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10S436/80
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

A molecule with two pendant phenylarsine moieties according to the general structural Formula (I) and tautomers, acids, and salts thereof:wherein: (i) R1 or R2, are each independently O−, S−, OR3 or SR3 with the provision that if either R1 or R2 is absent, the other remaining group is ═O or ═S; or R1 and R2, together with the arsenic atom, form a ring according to one of the general structural Formulae (II), (III), (IV), or (V):wherein R3 is H, CH(OH)CH2OH, or (CH2)q—Y, with q being 1–4 and Y being H, OH, NH2, SH, COOH, OAc, CONH2 or CN, and Z represents a hydrocarbon chain comprising 2–4 singly or doubly bonded carbon atoms each of which may be further substituted with one or more of hydrogen, methyl, ethyl, 1-propyl, 2-propyl, methoxy, hydroxy, amino, carboxy, sulfo, oxo, thio, halo (fluoro, chloro, bromo, or fluoro) and (CH2)n″SO3, wherein n″ is 1 or 2; (ii) R4, R5, R6 and R7 are each independently H, F, OR3, R3, OAc, NH2, N(C1–C4 alkyl)2, R1; or R4 with R5, or R6 together with R7, or both, form a ring; (iii) R8 is a linear or branched optionally substituted spacer having a minimum length of approximately 1.5 and a maximum length of approximately 15 Ångstroms; and (iv) X is a de…