Recombinant polypeptides having Ph1 p I epitope antigenicity and methods of diagnosing allergy in vitro

US7148019B2 · kind B2 · utility

Assignee

• PHADIA AB · SE

Inventors

• Tanja Ball · Gleisdorf, AT
• Susanne Vrtala · Wien, AT
• Wolfgang Sperr · Wien, AT
• Peter Valent · Wien, AT
• Markus Susani · Salzburg, AT
• Dietrich Kraft · Wien, AT
• Rudolf Valenta · Theresienfeld, AT
• Sylvia Laffer · Wien, AT

Key dates

• Filing date: Jun 5, 2002
• Grant date: Dec 12, 2006
• Expiry date: Oct 1, 2023

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K2319/00
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

A recombinant DNA molecule comprising a nucleotide sequence (I) which codes for a polypeptide displaying the antigenicity of one, two or more of the Phl p I epitope clones (28, 34, 41, 42, 43, 45, 50, 52, 64, 80, 85, 86, 95, 97, 98, 103, 108, 109, 113, 114), with the amino acid sequences defined in SEQ ID NOS: 5, 7, 9 and 12–28, and preferably being derived from grasses or monocotyledonic plants, or a nucleotide sequence (II) which hybridizes with such a nucleotide sequence (I) under conditions of high, stringency. Polypeptides displaying the antigenicity of one, two or more of the Phl p I epitope clones (28, 34, 41, 42, 43, 50, 52, 64, 80, 85, 86, 95, 97, 98, 103, 108, 109, 113, 114). Recombinant expression vectors containing the recombinant molecule and host cells transformed with the vector. Diagnostic methods based on utilizing the polypeptides in immunoassays for humoral antibodies and cellular reactions.