Heterologous G protein coupled receptors expressed in yeast, their fusion with G proteins and use thereof in bioassay

US7148053B2 · kind B2 · utility

Assignee

• BASF SE

Inventors

• Mark Henry Pausch · Lawrence, US
• Bradley A. Ozenberger · Yardley, US
• John R. Hadcock · Mount Holly, US
• Laura Price · Langhorne, US
• Eileen Kajkowski · Ringoes, US
• Donald R. Kirsch · Princeton, US
• Deborah T. Chaleff · Pennington, US

Key dates

• Filing date: Nov 25, 2002
• Grant date: Dec 12, 2006
• Expiry date: Jan 24, 2024

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K2319/00
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Expression vectors and yeast cells that contain a heterologous G protein-coupled receptor gene and a gene mutation that causes increased sensitivity to receptor activation or a gene mutation that permits transcriptional activation of pheromone-responsive genes without cell cycle arrest. Methods of making the yeast cells.