Temperature sensitive mutant of bacteriophage T4 endonuclease VII
US7148339B2 · kind B2 · utility
1Cited by
7References
4Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Mar 23, 2005 |
| Grant date | Dec 12, 2006 |
| Priority date | — |
| Expiry date | Mar 23, 2025 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/85
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The subject invention provides for a method of selectively cloning homoduplex nucleic acid molecules, in particular, by using a strain of host cells that contains a conditionally expressed and/or conditionally active mismatch-recognizing enzyme, e.g., a temperature sensitive variant of the gene encoding the endonuclease VII from phage T4. Using this host strain, the invention features a novel cloning method that selects for PCR products that are devoid of PCR-generated mutations.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.