RNA detection assays

US7150982B2 · kind B2 · utility

Assignee

• Third Wave Technologies, Inc. · US

Inventors

• Hatim Allawi · Madison, US
• Brad T. Argue · Sun Prairie, US
• Christian Bartholomay · Madison, US
• LuAnne Chehak · Janesville, US
• Michelle Curtis · Cottage Grove, US
• Peggy S. Eis · Fitchburg, US
• Jeff G. Hall · Madison, US
• Hon Ip · Madison, US
• Lin Ji · Sugar Land, US
• Michael W. Kaiser · Madison, US
• Robert Kwiatkowski · Verona, US
• Andrew A. Lukowiak · Stoughton, US
• Victor Lyamichev · Madison, US
• Natalie E. Lyamicheva · Madison, US
• WuPo Ma · Madison, US
• Bruce Neri · Madison, US
• Sarah Olson · Middleton, US
• Marilyn Olson-Munoz · Madison, US
• James J. Schaefer · Madison, US
• Zbigniev Skrzypczynski · Verona, US
• Tsetska Y. Takova · Madison, US
• Lisa C. Thompson · Madison, US
• Kevin L. Vedvik · Sun Prairie, US

Key dates

• Filing date: Feb 26, 2002
• Grant date: Dec 19, 2006
• Expiry date: Dec 15, 2022

Classification

• Technology area (CPC H): Electricity
• CPC primary: H04L9/40
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present invention provides novel cleavage agents and polymerases for the cleavage and modification of nucleic acid. The cleavage agents and polymerases find use, for example, for the detection and characterization of nucleic acid sequences and variations in nucleic acid sequences. In some embodiments, the 5′ nuclease activity of a variety of enzymes is used to cleave a target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.