Method for in vitro molecular evolution of protein function involving the use of exonuclease enzyme and two populations of parent polynucleotide sequence

US7153655B2 · kind B2 · utility

Assignee

• ALLIGATOR BIOSCIENCE AB · SE

Inventors

• Carl Arne Krister Borrebaeck · Lund, SE
• Ann-Christin Malmborg-Hager · Helsingborg, SE
• Christina Furebring · Lund, SE
• Ulf Hans Eskil Soderlind · Lund, SE
• Rebecka Ingrid Camilla Ottosson · Lund, SE

Key dates

• Filing date: Dec 17, 2002
• Grant date: Dec 26, 2006
• Expiry date: Jun 16, 2024

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N15/1027
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present invention relates to a method for in vitro evolution of protein function. In particular, the method relates to the shuffling of nucleotide segments obtained from exonuclease digestion. The present inventors have shown that polynucleotide fragments derived from a parent polynucleotide sequence digested with an exonuclease can be combined to generate a polynucleotide sequence which encodes for a polypeptide having desired characteristics. This method may be usefully applied to the generation of new proteins (e.g., antibodies and enzymes) or parts thereof having modified characteristics as compared to the parent protein.