Microfluidic devices and methods for performing temperature mediated reactions
US7153673B2 · kind B2 · utility
Assignee
Inventor
Key dates
| Filing date | Sep 30, 2003 |
| Grant date | Dec 26, 2006 |
| Priority date | — |
| Expiry date | Feb 16, 2025 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q2565/629
- WIPO fieldChemical engineering
- WIPO sectorChemistry
Abstract
In a method for performing a hot start polymerase chain reaction, a reaction mixture including a primer, a template molecule, and a buffer—but no polymerase enzyme—is loaded in a reaction channel of a microfluidic device. An electrical current is applied to heat the reaction channel. A polymerase enzyme is delivered into the reaction channel, and the reaction channel is subjected to conditions such that the hot start polymerase chain reaction is performed. In a method for performing a temperature mediated reaction, a first component of the temperature mediated reaction is loaded in a reaction channel of a microfluidic device. An electrical current is applied to heat the reaction channel. A second component of the temperature mediated reaction is delivered into the reaction channel, and the reaction channel is subjected to conditions such that the temperature mediated reaction is performed.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.