Method to detect the end-point for PCR DNA amplification using an ionically labeled probe and measuring impedance change

US7157232B2 · kind B2 · utility

Assignee

• The Regents of the University of California · US

Inventors

• Robin R. Miles · Danville, US
• Phillip Belgrader · Severna Park, US
• Christopher Fuller · Oakland, US

Key dates

• Filing date: Dec 21, 2004
• Grant date: Jan 2, 2007
• Expiry date: Aug 29, 2025

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/6823
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Impedance measurements are used to detect the end-point for PCR DNA amplification. A pair of spaced electrodes are located on a surface of a microfluidic channel and an AC or DC voltage is applied across the electrodes to produce an electric field. An ionically labeled probe will attach to a complementary DNA segment, and a polymerase enzyme will release the ionic label. This causes the conductivity of the solution in the area of the electrode to change. This change in conductivity is measured as a change in the impedance been the two electrodes.