High resolution imaging fountain flow cytometry
US7161665B2 · kind B2 · utility
Assignee
Inventor
Key dates
| Filing date | May 19, 2004 |
| Grant date | Jan 9, 2007 |
| Priority date | — |
| Expiry date | Jun 17, 2025 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N15/1433
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
An imaging fountain flow cytometer allows high resolution microscopic imaging of a flowing sample in real time. Cells of interest are in a vertical stream of liquid flowing toward one or more illuminating elements at wavelengths which illuminate fluorescent dyes and cause the cells to fluoresce. A detector detects the fluorescence emission each time a marked cell passes through the focal plane of the detector. A bi-directional syringe pump allows the user to reverse the flow and locate the detected cell in the field of view. The flow cell is mounted on a computer controlled x-y stage, so the user can center a portion of the image on which to zoom or increase magnification. Several computer selectable parfocal objective lenses allow the user to image the entire field of view and then zoom in on the detected cell at substantially higher resolution. The magnified cell is then imaged at the various wavelengths.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.