Process for isolating sea cucumber saponin Frondoside A, and immunomodulatory methods of use
US7163702B1 · kind B1 · utility
Inventors
Key dates
| Filing date | Jun 6, 2005 |
| Grant date | Jan 16, 2007 |
| Priority date | — |
| Expiry date | Jun 6, 2025 |
Classification
- Technology area (CPC A)Human Necessities
- CPC primaryA61P37/04
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
This present invention discloses use of a chloroform/solvent mixture extraction at different solvent-to-feed ratios, followed by evaporation and extraction with ethyl acetate/water; followed by chromatography of the water phase in Teflon or other non-polar resin and Silica gel column chromatography for recovering of individual triterpene glycosides (saponins) of high purity from the freeze dried or spray dried cooking water or dried powderized tissues of the industrial processed sea cucumber Cucumaria frondosa. A resulting glycoside Frondoside A stimulates lysosomal activity of peritoneal macrophages, phagocytosis and oxidative burst in the macrophages at concentrations significantly less than for acute toxicity, hemolysis and sea urchin embryo toxicity.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.