Method for characterizing polypeptides
US7163803B2 · kind B2 · utility
21Cited by
4References
22Claims
0Family size
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Key dates
| Filing date | Jun 7, 2002 |
| Grant date | Jan 16, 2007 |
| Priority date | — |
| Expiry date | Jun 7, 2022 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/6821
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Provided is a method for characterizing a polypeptide, which method comprises the steps of: (a) optionally reducing cysteine disulphide bridges in the polypeptide to form free thiols, and capping the free thiols; (b) cleaving the polypeptide with a sequence specific cleavage reagent to form peptide fragments; (c) optionally deactivating the cleavage reagent; (d) capping one or more ε-amino groups that are present with a lysine reactive agent; (e) analyzing peptide fragments by mass spectrometry to form a mass fingerprint for the polypeptide; and (f) determining the identity of the polypeptide from the mass fingerprint.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.