Fragments of fluorescent proteins for protein fragment complementation assays
US7166424B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Dec 1, 2003 |
| Grant date | Jan 23, 2007 |
| Priority date | — |
| Expiry date | Dec 1, 2023 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2333/43595
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present invention is directed to Protein-fragment Complementation Assays (PCAs) and assay compositions based on fluorescent proteins. The invention provides methods for fragmenting fluorescent proteins and generating mutant fragments with desired spectral characteristics for PCA. The invention encompasses assays and compositions based on fluorescent proteins from the species Aequorea, Anemonia and Anthozoa. In particular, the invention is directed to fragments of mutant fluorescent proteins having improved spectral properties over the wild-type proteins. The invention encompasses fragments of mutant versions of A. Victoria green fluorescent protein (GFP), in particular yellow fluorescent proteins (EYFP and super-EYFP), ‘Venus’, cyan, ‘citrine’, blue, cyan-green, and photoactivatable variants of GFP.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.