Detection of nucleic acid sequence differences using coupled ligase detection and polymerase chain reactions
US7166434B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | May 12, 2004 |
| Grant date | Jan 23, 2007 |
| Priority date | — |
| Expiry date | May 12, 2024 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q2600/16
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to the detection of nucleic acid sequence differences using coupled ligase detection reaction and polymerase chain reaction. One aspect of the present invention involves use of a ligase detection reaction coupled to a polymerase chain reaction. Another aspect of the present invention relates to the use of a primary polymerase chain reaction coupled to a secondary polymerase chain reaction coupled to a ligase detection reaction. A third aspect of the present invention involves a primary polymerase chain reaction coupled to a secondary polymerase chain reaction. Such coupling of the ligase detection reaction and the polymerase chain reaction permits multiplex detection of nucleic acid sequence differences.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.