Transferrin assay

US7166473B2 · kind B2 · utility

Assignee

• AXIS-SHIELD AS · NO

Inventors

• Erling Sundrehagen · Oslo, NO
• Asgeir Husa · Oslo, NO
• Ingar Eilertsen · Oslo, NO

Key dates

• Filing date: Jun 10, 2002
• Grant date: Jan 23, 2007
• Expiry date: Jun 13, 2023

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10S436/811
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

The invention relates to an assay method for assessing a transferrin variant or combination of transferrin variants for the diagnosis and monitoring of alcoholism, and to kits for performing the assay. In particular, the invention provides a method of producing an algorithm for determining the content of a transferrin variant or combination of transferrin variants, preferably a CDT variant or combination of CDT variants, in a sample of body fluid, said method comprising: (a) obtaining at least two solutions each having known contents of asialo- (A1, A2, A3, etc.) and disialo-transferrins (D1, D2, D3, etc.); (b) determining the content in each of said solutions of a transferrin variant or combination of transferrin variants fractions substantially free from tri- and higher sialylated transferring; (c) determining the total transferrin variant content (T1, T2, T2, etc.) of said fractions; and (d) producing an algorithm capable of determining the content of any transferrin variant or combination of transferrin variants, preferably a CDT variant or combination of CDT variants, in any given sample of body fluid subjected to said determination step b).