Apparatus and separation media for separating particles in free-flow electrophoresis

US7169278B2 · kind B2 · utility

Assignee

• TECAN TRADING AG · CH

Inventors

• Christoph Eckerskorn · Ottobrunn, DE
• Hans-Jorg Grill · Männedorf, CH
• Gerhard Weber · Landsberg am Lech, DE
• Peter Weber · Altensteig, DE
• David Arthur Yost · Los Altos, US

Key dates

• Filing date: Jan 17, 2003
• Grant date: Jan 30, 2007
• Expiry date: Apr 14, 2024

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N27/44769
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

A free-flow electrophoresis (FFE) apparatus and separation medium for separating particles therefore, includes a separation chamber with a dosage pump for conveying medium, electrodes for applying an electric field in the medium and sample injection points for adding a mixture of particles to be separated and fractionation points for removing the particles. Definable charge labels couple with the particles to form charge-modified particles which display with a different migratory behavior in FFE The apparatus includes focusing buffers between the separation medium and at least one electrode which has much higher electrical conductivity than the separation medium.