Method for producing and screening mass-coded combinatorial libraries for drug discovery and target validation
US7169563B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Apr 19, 2002 |
| Grant date | Jan 30, 2007 |
| Priority date | — |
| Expiry date | Sep 26, 2023 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2458/15
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Methods for identifying a member of a mass-coded molecular library, which is a ligand for a biomolecule and binds to the biomolecule at the binding site of a known second ligand for the biomolecule are described. The methods includes contacting a mass-coded molecular library with a biomolecule; separating the biomolecule-ligand complexes from the unbound members of the mass-coded molecular library; contacting the biomolecule-ligand complexes with a second ligand to dissociate biomolecule-ligand complexes in which the ligand binds to the biomolecule at the binding site of the second ligand, thereby forming biomolecule-second ligand complexes and dissociated ligands; separating the dissociated ligands and biomolecule-second ligand complexes; and determining the molecular mass of each dissociated ligand.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.