Enzyme immobilization for electroosmotic flow
US7172682B2 · kind B2 · utility
3Cited by
5References
36Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Jan 24, 2003 |
| Grant date | Feb 6, 2007 |
| Priority date | — |
| Expiry date | Apr 12, 2024 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K17/14
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Disclosed herein is a method and apparatus of immobilizing a biocatalyst on a microfluidic biochip for conducting reactions in the presence of electroosmotic flow. The biochip includes a polymer on its microfluidic flow surfaces, wherein the polymer includes a first substituent selected from ionic groups of the same polarity or precursors thereof, a second substituent that is a hydrophobic group, and a third substituent comprising an immobilized biocatalyst-or precursor thereof. The biochip can be used to conduct multiple sequential biocatalyzed reactions in the presence of electroosmotic flow.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.