Method for determining the concentration of thrombin inhibitors and kits therefor
US7172878B1 · kind B1 · utility
Assignee
Inventors
Key dates
| Filing date | Jan 28, 2000 |
| Grant date | Feb 6, 2007 |
| Priority date | — |
| Expiry date | Jan 28, 2020 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/56
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention relates to a method for determining the concentration of thrombin inhibitors in a non-turbid body liquid or a non-turbid extract from a body liquid. It comprises the following steps. The body liquid is taken from a living body, and the body liquid is subjected to a separation from the turbid matter, if necessary. To the non-turbid body liquid thus obtained are added a coagulation-inhibiting substance not interfering in the transformation prothrombin/active meizothrombin or Mtdesfgl, resp., a chromogenic or fluorogenic substrate not dissociable by active meizothrombin or Mtdesfgl, resp., and a substance dissociating prothrombin into meizothrombin or Mtdesfgl, resp., and as an optional prothrobmin. The thus obtained solution or mixture, resp., is subjected to a wavelength-selective light absorption or light emission measurement as a function of the time. From the reduction of the light absorption or light emission per time unit is determined the amount of the thrombin inhibitor included in the body liquid by comparison to previously determined standard curves.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.