Methods of optimizing antibody variable region binding affinity
US7175996B1 · kind B1 · utility
3Cited by
17References
6Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Oct 13, 2000 |
| Grant date | Feb 13, 2007 |
| Priority date | — |
| Expiry date | Oct 13, 2020 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K2317/92
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to compositions and methods of producing higher affinity molecules, and in particular, higher affinity antibodies. Specifically, the invention contemplates methods of mutagenesis to create a population of oligonucleotides coding for altered polypeptides that are expressed in a host cell and subsequently sequenced to determine the amino acid determinants conferring higher affinity.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.