Methods for generating double stranded DNA comprising a 3′ single stranded portion and uses of these complexes for recombination
US7176025B2 · kind B2 · utility
30Cited by
43References
32Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Mar 11, 2003 |
| Grant date | Feb 13, 2007 |
| Priority date | — |
| Expiry date | Jul 24, 2024 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/1096
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Methods of recombining nucleic acids are provided. In particular, methods for the production of partially double stranded nucleic acids comprising a 3′ overhang from an RNA target and use in methods of recombining polynucleotides is described. These methods do not require thermocycling. The present invention also provides methods of recombining and selection which allow for identification of proteins comprising improved or desired characteristics.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.