Protein-protein interactions and methods for identifying interacting proteins and the amino acid sequence at the site of interaction
US7176035B2 · kind B2 · utility
Assignee
Inventor
Key dates
| Filing date | Nov 13, 2001 |
| Grant date | Feb 13, 2007 |
| Priority date | — |
| Expiry date | Aug 5, 2022 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/6845
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The invention relates to protein-protein interactions and methods for identifying interacting proteins and the amino acid sequence at the site of interaction. Using overlapping hexapeptides that encode for the entire amino acid sequences of the linker domains of human P-glycoprotein gene 1 and 3 (HP-gp1 and HP-gp3), a direct and specific binding between HP-gp1 and 3 linker domains and intracellular proteins was demonstrated. The method of the present invention was further validated with Annexin. The present invention thus demonstrates a novel concept whereby the interactions between two proteins are mediated by strings of few amino acids with high and repulsive binding energies, enabling the identification of high affinity binding sites between any interacting proteins.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.