Transgenic animals expressing light-emitting fusion proteins and diagnostic and therapeutic methods therefor
US7176345B2 · kind B2 · utility
7Cited by
9References
11Claims
0Family size
Assignee
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Key dates
| Filing date | Nov 4, 2002 |
| Grant date | Feb 13, 2007 |
| Priority date | — |
| Expiry date | Jan 15, 2024 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2500/02
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
Light-generating fusion proteins having a ligand binding site and a light-generating polypeptide moiety and their use as diagnostics, in drug screening and discovery, and as therapeutics, are disclosed. The light-generating fusion protein has a feature where the bioluminescence of the polypeptide moiety changes upon binding of a ligand at the ligand binding site. The ligand may be, for example, an enzyme present in an environment only under certain conditions, e.g., ubiquitin ligase in a hypoxic state, such that the light-generating fusion protein is “turned on” only under such conditions.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.