Method for controlling ratio of proteins/lipids in crop seeds

US7176350B2 · kind B2 · utility

Assignee

• ZHEJIANG ACADEMY OF AGRICULTURAL SCIENCES · CN

Inventors

• Jinqing Chen · Xiamen, CN
• Chunxiu Lang · 马宅镇, CN
• Ruizhi Huang · 马宅镇, CN
• Zhanghu Hu · 马宅镇, CN
• Xhihong Liu · 马宅镇, CN

Key dates

• Filing date: May 8, 2002
• Grant date: Feb 13, 2007
• Expiry date: Jul 8, 2023

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N15/8251
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A method for altering the ratio of proteins/lipids in crop seeds utilizing the antisense gene, comprising the steps of: (1) cloning the gene for the phosphoenopyruvate carboxylase or acetyl-CoA carboxylase, or the fragment thereof; (2) constructing the corresponding antigene; (3) introducing the said antigene into the cells of the rapeseeds, so that the activity of the phosphoenopyruvate carboxylase or the acetyl-CoA carboxylase being important for the protein synthesis is changed, the flow direction of the common substrate, PEP, can be controlled, so that the proteins/lipids in the seeds is altered. The application can be used to alter the proteins/lipids in the crop seeds.