Direct radio-frequency detection of nucleotide hybridization at microelectrodes
US7183055B2 · kind B2 · utility
25Cited by
4References
16Claims
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Key dates
| Filing date | Oct 27, 2003 |
| Grant date | Feb 27, 2007 |
| Priority date | — |
| Expiry date | Aug 25, 2024 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC40B70/00
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Radio-frequency (RF) excitation is used for direct detection of hybridization events at microelectrodes with surface-attached DNA oligomers. A homodyne reflectometer operates on a high frequency carrier to detect the presence of a low-frequency modulation signal. Without non-linearities in an interface, the modulation signal is not impressed upon the carrier signal. As such, the reflectometer can sensitively measure changes in dielectric properties without interference from other sources of capacitance/resistance unrelated to the reaction at the surface.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.