Methods for isolation and labeling of sample molecules

US7183116B2 · kind B2 · utility

Assignees

• Institute for Systems Biology · US
• University of Washington · US
• Applera Corporation · US

Inventors

• Rudolf Aebersold · Zürich, CH
• Huilin Zhou · San Diego, US
• Beate Rist · Giesen, DE
• George J. Vella · Medway, US
• Subhasish Purkayastha · Acton, US
• Sasi Pillai · Littleton, US

Key dates

• Filing date: May 14, 2001
• Grant date: Feb 27, 2007
• Expiry date: May 14, 2021

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10T436/24
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

The invention provides methods for labeling a molecule by contacting a sample molecule with a solid support coupled to a chemical group comprising a cleavable functional group, one or more functional groups, and a reactive group for the sample molecule, under conditions allowing the sample molecule to covalently bind to the reactive group; and cleaving the cleavable functional group, thereby releasing the sample molecule comprising the one or more functional groups, which can be a tag. The invention also provides a solid support covalently coupled to a chemical group comprising a cleavable functional group, a mass spectrometry tag and a reactive group for covalently attaching a sample molecule, wherein the cleavable functional group, the tag and the reactive group are positioned relative to each other to allow transfer of the tag to the sample molecule upon cleavage of the cleavable functional group.