Genes for heat resistant enzymes of amino acid biosynthetic pathway derived from thermophilic coryneform bacteria
US7183403B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Mar 8, 2005 |
| Grant date | Feb 27, 2007 |
| Priority date | — |
| Expiry date | Mar 8, 2025 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Y604/01002
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A plurality of primer sets are designed based on a region where conservation at the amino acid level is observed among various microorganisms for known gene sequences corresponding to a gene coding for an enzyme of the L-amino acid biosynthetic pathway derived from Corynebacterium thermoaminogenes, preferably an enzyme that functions at a higher temperature compared with that of Corynebacterium glutamicum. PCR is performed by using the primers and chromosomal DNA of Corynebacterium thermoaminogenes as a template. The primers with which an amplification fragment has been obtained are used as primers for screening to select a clone containing a target DNA fragment from a plasmid library of chromosomal DNA of Corynebacterium thermoaminogenes.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.