Capture and detection of target nucleic acid in dipstick assays
US7186508B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Jul 6, 2001 |
| Grant date | Mar 6, 2007 |
| Priority date | — |
| Expiry date | Apr 18, 2022 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N30/94
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Use of helper probes in dipstick assays is described. In a dipstick assay to test for the presence of a target nucleic acid in a sample solution, the sample solution is connected with the contact end of the dipstick to cause the sample solution is contacted with the contact end of the dipstick to cause the sample solution to move by capillary action to a capture zone of the dipstick at which target nucleic acid is captured. The target nucleic acid may be captured at the capture zone by a capture probe capable of hybridising to the target nucleic acid. A labelled detection probe capable of hybridising to the target nucleic acid may be used to detect the target nucleic acid at the capture zone. A helper probe may be used to enhance the binding of the capture and/or detection probe to the target nucleic acid, thereby improving the sensitivity of target nucleic acid detection. Dipsticks and kits are also described.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.